Technique to identify the different varieties a sample of malt has
I have a laboratory of testing seed. I want to know, which technique do you use to identify the different varieties a sample of malt has.
I use electrophoresis of hordein proteins for barley, to verify the variety, but I do not have good results in malt. Can you help me?
Malt Variety Elucidation
The standard procedure for the identification of malting barley varieties is sodium dodecylsulfide polyacrilamide gel electrophoresis (SDS-page for short)of the hordein proteins.
However, it is not all inclusive. It really can only sort the pattern of bands into one of perhaps 20 patterns. So, for example, this technique cannot differentiate Stander from Robust.
Another technique, SDS-page of the esterase enzymes, can differentiate Stander and Robust.