yeast propagation

[fisherlandon]

I am opening a 3bbl brew pub and I am having some issues with yeast starting. I am wondering if anyone can lead me to a resource that can help me figure the amount of minimum amount of yeast to use in a 2-3bbl batch. my assumptions have been to get three 5 liter erlenmeyer flasks going on stir plates and then pitch. I am curious if this is even a possibility, I am open to anything I just need help with this for repeatability.

[lhall]

A rule of thumb is 1x10^6 cells/mL/ degree Plato.

[fa50driver]

http://www.brewingscience.com/PDF/BS...b_handbook.pdf

These guys are very helpful as well - they have always talked me through any issues.

[CaptainEBC]

We just buy a pitchable quantity from White-Labs. Then we use a microscope to determine cell count / amount to re-pitch for the next generations.

[coolguywalt]

+1 for the Brewing Science Institute. David Bryant and his folks are a great yeast resource!