Hello brewers and QC people,
As we expand from 10k bbl/year last year, 25k this year, and 45k next year, we're going to need some better micro checks. I am pricing out the differences in cost between plating and qPCR. I have a very extensive background in qPCR, but was always using mammalian cell kits. If any of you currently use qPCR, what lysis kits do you use for yeast/hopefully not but possible bacteria, and how do you quantify it? Do you have a ratio from Ct to CFU?
I think qPCR is the route to go, because it's a 40 min answer instead of 5 days. It's also a lot less labor. The capital cost is a lot though. I just need to find the right kits. I have primers for all the hop resistant strains already. halp!
Cheers!
As we expand from 10k bbl/year last year, 25k this year, and 45k next year, we're going to need some better micro checks. I am pricing out the differences in cost between plating and qPCR. I have a very extensive background in qPCR, but was always using mammalian cell kits. If any of you currently use qPCR, what lysis kits do you use for yeast/hopefully not but possible bacteria, and how do you quantify it? Do you have a ratio from Ct to CFU?
I think qPCR is the route to go, because it's a 40 min answer instead of 5 days. It's also a lot less labor. The capital cost is a lot though. I just need to find the right kits. I have primers for all the hop resistant strains already. halp!
Cheers!
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