Hi!
Took up yeast counting again been a long time since I did it.
I'm helping out a small brewery and have sourced the needed material.
I got methylene blue 2% solution which I have diluted with distilled
water to 0.5%.
I took 1ml of diluted yeast solution and 1ml water and finally 1ml 0.5% methylene blue solution with a total dilution
factor of 300. Added a drop to the hemocytometer and into the microscope with 400x magnification.
Now to my problem, all cells are blue and blue-ish..
Which concentration of methylene blue are you using?
How much do you usually dilute your samples?
I will redo counting and take a bigger sample from the yeast-brink
and see if that helps..
Cheers, R
Took up yeast counting again been a long time since I did it.
I'm helping out a small brewery and have sourced the needed material.
I got methylene blue 2% solution which I have diluted with distilled
water to 0.5%.
I took 1ml of diluted yeast solution and 1ml water and finally 1ml 0.5% methylene blue solution with a total dilution
factor of 300. Added a drop to the hemocytometer and into the microscope with 400x magnification.
Now to my problem, all cells are blue and blue-ish..
Which concentration of methylene blue are you using?
How much do you usually dilute your samples?
I will redo counting and take a bigger sample from the yeast-brink
and see if that helps..
Cheers, R
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