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Thread: Preparing Media and Autoclaving

  1. #1
    Join Date
    Apr 2018
    Location
    Kentwood, MI, USA
    Posts
    2

    Exclamation Preparing Media and Autoclaving

    Hello, I am preparing a lot of media today for plating later on. I was wondering if I am able to prepare the media for autoclaving and place the media into an un-autoclaved pyrex jar. It would be autoclaved immediately after. This theoretically would still sterilize the media and the glassware correct? Thoughts?

  2. #2
    Join Date
    Mar 2018
    Location
    Chicago, IL
    Posts
    4
    If i'm understanding correctly, you should be good. If you are dissolving the media in an un-autoclaved jar, and then autoclaving that jar with the dissolved media immediately after dissolving it, then yep it will all be sterile. Just need to let it cool down to ~60 dC before making your plates while keeping the lid slightly open to prevent a vacuum.

  3. #3
    Join Date
    Apr 2018
    Location
    Kentwood, MI, USA
    Posts
    2
    That's what I thinking but just wanted to hear some feedback before I pulled the trigger. Thank you

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