I just did a experiment on determining the amylase activity in germinating barley. This is done by measuring the rate of starch hydrolysis.
My problem is that I do not know how to get the rate.
The only thing is given is the concentration of the starch, the time take to reach achromic point and the volume of diluted amylase extract added to the buffer and starch solution.
So, I hope that you sir, can provide an answer to my question, cause I have looked everywhere for answer.
My problem is that I do not know how to get the rate.
The only thing is given is the concentration of the starch, the time take to reach achromic point and the volume of diluted amylase extract added to the buffer and starch solution.
So, I hope that you sir, can provide an answer to my question, cause I have looked everywhere for answer.
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