I'd like to use wlp007 as our house ale strain but due to the difficulty in trying to get a homogenous and non-clumpy sample to actually count cells, I finally tried using phosphoric acid (85%) I normally use to adjust mash ph.
I read about it here:
I tried it on a tube of wlp007 and it seemed to work great, floccs smoothed out and cells looked normal and not dead - however, it DID require me to dilute the sample with more acid than I normally would havee thought. My procedure for serial dilutions of slurry call for weighing out 40g of slurry and then adding to that slurry exactly (by weight in grams) the same amount of distilled water to create a 1:1 dilution.
My question is: if I used the phosphoric acid for the 1:1 dilution step, would that cause any issues as far as an accurate cell count? It seems to me that it wouldn't given that it's all based on exact weights anyway - any issues with this that you all can see?
I read about it here:
I tried it on a tube of wlp007 and it seemed to work great, floccs smoothed out and cells looked normal and not dead - however, it DID require me to dilute the sample with more acid than I normally would havee thought. My procedure for serial dilutions of slurry call for weighing out 40g of slurry and then adding to that slurry exactly (by weight in grams) the same amount of distilled water to create a 1:1 dilution.
My question is: if I used the phosphoric acid for the 1:1 dilution step, would that cause any issues as far as an accurate cell count? It seems to me that it wouldn't given that it's all based on exact weights anyway - any issues with this that you all can see?
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