Gram Staining
Hey, for what it's worth, a quick Gram stain of the unfiltered beer will give you an immediate indication of any presence of bacteria or some wild yeasts. I don't even go beyond the crystal violet step--you can pick out the individual cell shapes perfectly fine without safranin. Lactobacillus and Pediococcus are very distinctive, as well as Brettanomyces. This is the quickest and cheapest option as far as I've seen, and can help you eliminate those samples which do not raise any red flags before you go about molecular techniques like qPCR. Unfortunately, it will be necessary to use PCR when Diastaticus is suspected though.
Hey, for what it's worth, a quick Gram stain of the unfiltered beer will give you an immediate indication of any presence of bacteria or some wild yeasts. I don't even go beyond the crystal violet step--you can pick out the individual cell shapes perfectly fine without safranin. Lactobacillus and Pediococcus are very distinctive, as well as Brettanomyces. This is the quickest and cheapest option as far as I've seen, and can help you eliminate those samples which do not raise any red flags before you go about molecular techniques like qPCR. Unfortunately, it will be necessary to use PCR when Diastaticus is suspected though.
Comment