I'm in the unfortunate situation of dealing with several batches in a row going sour. We have acquired the HLP testing media and started testing some past and new batches expecting (almost wanting) to see the infamous teardrops on them. To our dismay, most results have fallen in the ambiguous "it might have LAB, it might not" conclusion.
Only one of the batches revealed Pediococcus damnosus convincingly. Some have clear looking tubes, but a white fluffy colony covers the top of the liquid. As far as I know, it can't be fungal since the cicloheximide is there to supress eukariote grow; however, Acinetobacter doesn't seem likely since none of the batches tasted like vinegar:
Other went full turbid, but have no discernible colonies:
Got to say that even the blanks form some sort of insoluble flocs that float around of variable size. Is there a guide somewhere to interpret these weird results? Particularly the veil colony of the top, since those batches doesn't taste bad... yet.
Only one of the batches revealed Pediococcus damnosus convincingly. Some have clear looking tubes, but a white fluffy colony covers the top of the liquid. As far as I know, it can't be fungal since the cicloheximide is there to supress eukariote grow; however, Acinetobacter doesn't seem likely since none of the batches tasted like vinegar:
Other went full turbid, but have no discernible colonies:
Got to say that even the blanks form some sort of insoluble flocs that float around of variable size. Is there a guide somewhere to interpret these weird results? Particularly the veil colony of the top, since those batches doesn't taste bad... yet.
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