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Viability and Methylene Blue Solution

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  • Viability and Methylene Blue Solution

    So I am trying to make sure I am properly testing for viability when harvesting and repitching. Some sources I read say that a .1% methylene blue solution is all that is needed. White Labs has a solution that takes the .1% methylene blue solution and dilutes it further with a glycine buffer of 10.6 pH .1M at 10ml methylene blue solution per 100ml. What is the glycine buffer for? These methods contain drastically different concentrations of methylene blue? Just looking for some clarification. Cheers


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