Hello, I am preparing a lot of media today for plating later on. I was wondering if I am able to prepare the media for autoclaving and place the media into an un-autoclaved pyrex jar. It would be autoclaved immediately after. This theoretically would still sterilize the media and the glassware correct? Thoughts?
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Preparing Media and Autoclaving
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If i'm understanding correctly, you should be good. If you are dissolving the media in an un-autoclaved jar, and then autoclaving that jar with the dissolved media immediately after dissolving it, then yep it will all be sterile. Just need to let it cool down to ~60 dC before making your plates while keeping the lid slightly open to prevent a vacuum.
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Originally posted by berniebutgereit View PostHello, I am preparing a lot of media today for plating later on. I was wondering if I am able to prepare the media for autoclaving and place the media into an un-autoclaved pyrex jar. It would be autoclaved immediately after. This theoretically would still sterilize the media and the glassware correct? Thoughts?I hope I encouraged you!
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