I just got a new microscope and am trying to implement viability staining into my cell counting routine, but I'm running into some confusion. Depending on the focus of my microscope, even if I adjust it ever so slightly, my cells look either all clear or all dark blue! I know that there's no way I have 100% viability, or 100% dead cells. Has anyone else ever run into this problem?
I am using a 1:1000 yeast dilution (made via serial dilutions), a 0.1% MB dilution (made from 1 mL of 1% MB into 99 mL distilled water), and mixing 1 mL of the diluted yeast solution with 1 mL of the MB for about 2 minutes. I've also tried a 0.01% MB solution, with the same problem.
Thanks!
I am using a 1:1000 yeast dilution (made via serial dilutions), a 0.1% MB dilution (made from 1 mL of 1% MB into 99 mL distilled water), and mixing 1 mL of the diluted yeast solution with 1 mL of the MB for about 2 minutes. I've also tried a 0.01% MB solution, with the same problem.
Thanks!
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